REPORTS tion, we mutated this position in the PGT minigene. As seen in Fig. 4B (lanes 4 and 5), this point mutation was enough to activate the nearly constitutive inclusion of the Alu exon in the mature transcript. As in

Source URL: meiosis.cornell.edu

iCLIP Predicts the Dual Splicing Effects of TIA-RNA Interactions Zhen Wang1., Melis Kayikci1., Michael Briese1, Kathi Zarnack2, Nicholas M. Luscombe2,3, Gregor Rot4, Blazˇ Zupan4, Tomazˇ Curk4, Jernej Ule1* 1 Medical R

Source URL: www.ulelab.info

• Biology
• Genetics
• Gene expression
• Spliceosome
• RNA splicing
• Molecular biology
• RNA
• Alternative splicing
• Minigene
• CLIP
• RNA-binding protein
• Exon

TIGS-863; No. of Pages 9 Review Understanding splicing regulation through RNA splicing maps

Source URL: www.ulelab.info

• Biology
• RNA
• Genetics
• Gene expression
• Spliceosome
• Molecular biology
• Alternative splicing
• RNA-binding protein
• Minigene
• RNA splicing
• SR protein
• Intron

Finding Characteristic Sequence Patterns for Alternative Splicing in Human Genomic DNA Hiroki Sakai1 Osamu Maruyama2

Source URL: www.jsbi.org

• Genetics
• Biology
• Spliceosome
• RNA splicing
• Gene expression
• DNA
• Alternative splicing
• Exon
• Intron
• Exonic splicing enhancer
• Minigene

1 Supporting Online Materials Materials and Methods Plasmid Constructs Oligonucleotide primers were designed to amplify (from human genomic DNA) a minigene that contains exons 7, 8, and 9 of the adenosine deaminase (ADAR

Source URL: www.tau.ac.il

• Molecular biology
• Polymerase chain reaction
• Laboratory techniques
• DNA
• RNA splicing
• Reverse transcription polymerase chain reaction
• Site-directed mutagenesis
• Complementary DNA
• Primer
• Biology
• Genetics
• Biochemistry