DEVELOPMENT OF A PROTOPLAST SYSTEM FOR NON-TRANSGENIC, TARGETED GENOME EDITING IN S. TUBEROSUM Fossi M.M.1,2,3, Amundson K.R.1,2, Jinata S.N.1, Comai L.1,2 1Genome Center, 2Plant Biology Department, University of Califo

Source URL: www.seedcentral.org

Studies on Regulatory Mechanisms of Pupal Metamorphosis in the Silkworm Using Genomics and Genome Editing. Takaaki Daimon (Graduate School of Agriculture, Kyoto University) Insect molting

Source URL: www.nougaku.jp

Matthew Billman HumBio 158G Douglas Brutlag, Instructor Genome Editing Techniques for Treatment of HIV We live in an exciting time. Knowledge of the human genome increases yearly at an exponential rate, vastly exceeding

Source URL: biochem158.stanford.edu

GENOME EDITING OUTLOOK PERSPECTIVE Encourage the innovators I

Source URL: arep.med.harvard.edu

Genome Editing Are plants engineered with CRISPR technology genetically modified organisms?

Source URL: www.biochemist.org

2A-linked TALEN assembly protocol PROTOCOL FOR: An improved self-cleaving peptide-linked TALEN scaffold for efficient genome editing Andrew Mariano, Li Xu and Renzhi Han

Source URL: static-content.springer.com

A general protocol of NgAgo/gDNA-mediated genome editing Cell culture 293T cells are maintained in high-glucose DMEM (Gibco) supplemented with 10% FBS (Gibco) and penicillin/streptomycin at 37°C with 5% CO2 incubation.

Source URL: www.addgene.org

Therapeutic Correction of an LCA-Causing Splice Defect in the CEP290 Gene by CRISPR/Cas-Mediated Genome Editing Morgan L. Maeder, Rina Mepani, Sebastian W. Gloskowski, Maxwell N. Skor, McKensie A. Collins, Gregory M. Got

Source URL: www.editasmedicine.com

• Genetics
• Biology
• Spliceosome
• Gene expression
• RNA splicing
• DNA
• Genetic engineering
• Exon
• CEP290
• CRISPR
• Cas9
• Guide RNA

5560–5568 Nucleic Acids Research, 2012, Vol. 40, No. 12 doi:nar/gks179 Published online 28 FebruaryEngineered zinc finger nickases induce

Source URL: www.ncbi.nlm.nih.gov

• Biology
• DNA repair
• Molecular biology
• Genetic engineering
• Biotechnology
• Telomeres
• Zinc finger nuclease
• Genome editing
• Transcription activator-like effector nuclease
• Restriction enzyme
• Non-homologous end joining
• Daniel Voytas

Therapeutic Correction of an LCA-Causing Splice Defect in the CEP290 Gene by CRISPR/Cas-Mediated Genome Editing Morgan L. Maeder, Rina Mepani, Sebastian W. Gloskowski, Maxwell N. Skor, McKensie A. Collins, Gregory M. Got

Source URL: www.editasmedicine.com

• Genetics
• Biology
• Spliceosome
• Gene expression
• DNA
• RNA splicing
• Genetic engineering
• Cas9
• Exon
• CRISPR
• CEP290
• Intron