Molecular cloning, sequence characterization, and gene expression profile of a novel water buffalo (Bubalus bubalis) gene: Na+, K+-ATPase β2-subunit (ATP1B2)

Source URL: doi.fbn-dummerstorf.de

    Transfection  and  Subsequent  Transduction  Protocols  for  Single  shRNA  Expression   Constructs  From  TRC1,  1.5,  or  2  Library  Clones    

Source URL: functionalgenomicsfacility.org

• Biology
• Molecular biology
• Biochemistry
• Gene delivery
• Applied genetics
• Biotechnology
• Genetics
• Transfection
• Puromycin
• Short hairpin RNA
• Molecular cloning
• HEK 293 cells

The MultiBac BEVS for producing proteins and their complexes (Prot54) The MultiBac BEVS for producing proteins and their complexes (Prot54) Frederic Garzoni, Christoph Bieniossek and Imre Berger

Source URL: www.epigenesys.eu

• Molecular biology
• Gene delivery
• Plasmid
• Expression vector
• Transformation
• Vector
• Fertility factor
• Transfection
• Bacterial artificial chromosome
• Recombineering
• Molecular cloning
• Viral vector

IFAFS Project Milestones Pituitary Breast

Source URL: cogburn.dbi.udel.edu

• Molecular biology
• DNA
• Genetics
• Gene expression
• Genomics
• Expressed sequence tag
• Library
• CDNA library
• Quantitative trait locus
• Complementary DNA
• Molecular cloning

CRB-FUNDED RESEARCH PROGRESS REPORT Figure 1. In order to find nucleotide variability at both ends (3’ and 5’) of CTV California strain genome (highlighted in gray), the virus RNA has to be converted to a double-stra

Source URL: citrusresearch.org

• Molecular biology
• Citrus tristeza virus
• Complementary DNA
• Expressed sequence tag
• Transcription
• Genome
• Sequencing
• Molecular cloning
• Gene expression
• RNA
• DNA
• DNA sequencing

Yeast Two-Hybrid Procedure 1. Generate a GAL4 DNA-BD fusion by cloning the gene of interest in frame with the GAL4 DNA binding domain of pGBKT7. 2. Transform AH109 with bait plasmid, detect bait expression and test for a

Source URL: hanlab.xmu.edu.cn

• Laboratory equipment
• Agar
• Gels
• Polysaccharides
• Red algae
• Laboratory centrifuge

Frequently Asked Questions (CCSB LentiORF) General Information about CCSB-Broad Lentiviral Expression Library Disclaimer The Functional Genomics Facility is a distributor of LentiORF (clone ID. eg. ccsb304_xxxx) collecti

Source URL: functionalgenomicsfacility.org

• Molecular biology
• Molecular genetics
• The RNAi Consortium
• Open reading frame
• RNA interference
• Lentivirus
• Vesicular stomatitis virus
• Addgene
• Vector
• Dharmacon
• Inc.
• Expression cloning
• Library

  Adaptation  of  the  pET-­‐Duet  expression  system  to  USER-­‐Fusion   based  cloning  -­‐  using  statin  biosynthesis  as  a  case  study   Malgorzat

Source URL: files.conferencemanager.dk

DNA cloning for protein expression using Gateway© technology Artem Evdokimov 1. Reagents & their properties. Storage & propagation of Gateway components. Helpful things to be aware of regarding storage a

Source URL: www.xtals.org

A PCR-based strategy for cloning short hairpin sequences: “PCR SHAGging”. Our overall approach is to use an RNA polymerase III promoter to drive expression of encoded short hairpin RNA (shRNA). For this purpose we us

Source URL: hannonlab.cshl.edu