Transfection

Results: 295



#Item
31

Sugden Lab. Transfection by Lipofectamine (using Invitrogen LipofectamineTM) 1. The day before transfection plate cells into 6 cm dish so that they are 80~90% confluent

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Source URL: mcardle.oncology.wisc.edu

Language: English - Date: 2006-09-27 17:40:14
    32Biology / Biotechnology / Cell biology / Cell culture / Microfluidics / Nanotechnology / Microfluidic cell culture / Subculture / Flow cytometry / HeLa / Tissue engineering / Transfection

    Micro- & nano- fluidic research for chemistry, physics, biology, & bioengineering www.rsc.org/loc Volume 10  |  Number 12  |  21 June 2010  |  Pages 1493–1632 ISSN

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    Source URL: microfluidics.utoronto.ca

    Language: English - Date: 2011-08-09 14:23:18
    33

    Creating Cell-Based Assays for Screening GPCRs, Ion Channels and Other Targets in Cell Lines and Primary Cells Using the MaxCyte® STX™ Scalable Transient Transfection System James Brady1, Cornell Allen1, Rama Shivakum

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    Source URL: www.maxcyte.com

    Language: English - Date: 2013-08-02 13:00:51
      34

      Low-frequency ac electroporation shows strong frequency dependence and yields comparable transfection results to dc electroporation

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      Source URL: www.microfluidics.che.vt.edu

      Language: English - Date: 2012-05-01 10:47:06
        35

        Sugden Lab. Transfection by Electroporation Want the cells to be growing at 5 x 10^5. Want 5 x 10^6 cells per transfection. The total volume is 500 ul per transfection

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        Source URL: mcardle.oncology.wisc.edu

        - Date: 2006-09-27 17:40:22
          36

          Sugden Lab. Preparation for Retroviral vector Generating retrovirus particles 1. Plate 293T cells to 10 cm plates with a 1:8~10 split so that they are ~60% confluent the day of transfection.

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          Source URL: mcardle.oncology.wisc.edu

          Language: English - Date: 2006-09-27 17:40:06
            37

            Transfection of Sf9 cells in suspension December, 2008 Version 1.0 Summary: This protocol is used to generate baculoviruses containing GPCR genes of interest for expression in SF9 cells. This protocol is a slight

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            Source URL: jcimpt.usc.edu

            Language: English - Date: 2008-12-29 18:08:53
              38

              IP of AMPK for kinase assay After transfection of cells is complete: Remove media from plate Add 1ml lysis buffer to plate. Scrape cells and transfer to a fuge tube. Keep on ice for 10 min. Spin for 5 min @ 14000g @

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              Source URL: www.med.upenn.edu

              Language: English
                39Gene delivery / Gene expression / PAX6 / Molecular neuroscience / Glycoproteins / Vector / Reporter gene / Promoter / Transfection / Biology / Biochemistry / Molecular biology

                Journal of Neurochemistry, 2007, 103, 650–665 doi:j04797.x Department of Psychiatry, College of Medicine, University of Illinois at Chicago, Psychiatric Institute, Chicago, Illinois, USA

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                Source URL: champagnelab.psych.columbia.edu

                Language: English - Date: 2011-01-25 18:53:45
                40Transformation / Molecular cloning / Library / CDNA library / Transfection / Lipofectamine / Vector / Complementary DNA / Plasmid / Biology / Molecular biology / Biochemistry

                Microsoft Word - TrueCLONE Application Guide711.docx

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                Source URL: www.origene.com

                Language: English - Date: 2013-01-16 14:25:50
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