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City Scientific / Dresden / Bewersdorf / Paris / Oxford / Berlin / Orsay / London / San Diego / FRAISIER ET / Rietdorf / / Company 3D CSD / Gould / Academic Press / Brown / Nikon / MatTek Corporation / YZ. / Multidimensional / Mad City Labs / Wilson / Roper / University Press / Stack / J A S A / BD Biosciences / Ubq / Yokogawa / / Country France / Japan / United States / United Kingdom / / Currency pence / / / Facility Nikon Imaging Centre / Scale bar / Indicated bar / France ‡Max Planck Institute of Molecular Cell Biology / / IndustryTerm immersion oil / image-based iterative algorithms / retinal imaging / live material imaging / satellite images / oil / oil 10S / imaging / iterative algorithms / oil objective / live imaging / biological samples imaging / fixed and live cell imaging / confocal imaging / image-based iterative algorithm / oil immersion objectives / 3N algorithm / biological imaging / cellular imaging / high numerical aperture oil immersion objective / disk device / / OperatingSystem L3 / / Organization France ‡Max Planck Institute of Molecular Cell Biology and Genetics / Nikon Imaging Centre / CNRS Institut Curie / Royal Microscopical Society / Germany §Institut Curie / European Union / / Person Maria-Alexandra Rujano Maldonado / Nat / Julie Plastino / Dora Sabino / Jean Salamero / / / Position permeabilization buffer 1h RT / paraformaldehyde RT / RT / Major / / ProgrammingLanguage C / / ProvinceOrState Alabama / P.N. / California / / PublishedMedium Plos One / / Technology 3N algorithm / 3-D / fiber optic / Then image-based iterative algorithms / Gibco Life technologies / laser / lasers / Spectroscopy / simulation / Image-based iterative algorithms / image-based iterative algorithm / / SocialTag