Plasmids

Results: 41



#Item
11Cell signaling / Growth factors / Cell communication / Platelet-derived growth factor / Mitogen-activated protein kinase / Cell surface receptor / Cell / Biology / Cell biology / Signal transduction

Supplemental Information EXTENDED EXPERIMENTAL PROCEDURES Plasmids and Constructs Used All constructs for mammalian expression were cloned into the pHR lentiviral backbone (kindly provided by John R. James). PhymCherry-C

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Source URL: limlab.ucsf.edu

Language: English - Date: 2014-12-24 04:43:02
12Cytoskeleton / Gene delivery / Mobile genetic elements / ParM / R1 plasmid / Plasmid / Fertility factor / Biology / Microbiology / Molecular biology

Micro201 Rudner Lecture 2: plasmid segregation 26 March 2015 Overview: In this class we will discuss plasmids and their segregation. The background readings include a comprehensive review on this topic (Ebersbach and Ger

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Source URL: micro.med.harvard.edu

Language: English - Date: 2015-03-22 13:48:42
13Bacteriology / Molecular biology / Gene delivery / Plasmid / FtsZ / Fertility factor / Actin / Cell / Chromosome / Biology / Microbiology / Cytoskeleton

Movement and equipositioning of plasmids by ParA filament disassembly Simon Ringgaarda,b, Jeroen van Zonc,d, Martin Howarde, and Kenn Gerdesa,1 aCentre for Bacterial Cell Biology, Institute for Cell and Molecular Biosci

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Source URL: micro.med.harvard.edu

Language: English - Date: 2015-03-22 13:48:51
14

Supplementary Materials and Methods Plasmids and Reagents MitoTracker® Red CM-H2XRos was purchased from Life Technologies. The HIF-response element (HRE)-luciferase plasmid was obtained from Affymetrix (Santa Clara, CA,

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Source URL: cancerandmetabolism.com

Language: English
    15Physical geography / Sea ice / Aquatic ecology / Polar ice packs / Ice core / Ice / Drift ice / Southern Ocean / Glaciology / Water / Earth

    nonrecoverable, state with time of exposure (figure 1). These results indicate antibiotic-resistance and conjugative plasmids are maintained and expressed in E. coli exposed to the antarctic marine environment. In additi

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    Source URL: s3.amazonaws.com

    Language: English - Date: 2011-05-06 18:41:48
    16Genetics / Molecular genetics / DNA ligase / DNA replication / Guide RNA / Nuclear localization sequence / Transformation / DNA / Ligation / Biology / Molecular biology / RNA

    CRISPR plasmids from Chen and Wente labs We have synthesized a Cas9 coding sequence with nuclear localization signals (nls) at both its amino and carboxyl termini and codons optimized for zebrafish expression (nlszCas9-n

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    Source URL: www.addgene.org

    Language: English - Date: 2014-11-05 23:32:00
    17Gene delivery / Microbiology / Mobile genetic elements / Plasmid / Vector / Caenorhabditis elegans / Biology / Molecular biology / Biochemistry

    Fire Lab C. elegans Vector Kit Description: The Andrew Fire laboratory has deposited a set of 288 vectors for C. elegans research. The plasmids are divided into three plates: 1995 plate, 1997 plate, and 1999 plate. 24 ve

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    Source URL: www.addgene.org

    Language: English - Date: 2014-11-05 23:32:07
    18Signal transduction / Wnt signaling pathway / WNT7B / WNT3A / WNT1 / WNT3 / WNT7A / WNT4 / Plasmid / Biology / Biochemistry / Genes

    Open Source Wnt Kit Description: The Open Source Wnt Project was initiated to create a collection of standardized Wnt plasmids that are easily accessible to the research community. The project and plasmids were created b

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    Source URL: www.addgene.org

    Language: English - Date: 2014-11-05 23:31:57
    19Genetics / Molecular genetics / DNA ligase / DNA replication / Guide RNA / Nuclear localization sequence / Transformation / DNA / Ligation / Biology / Molecular biology / RNA

    CRISPR plasmids from Chen and Wente labs We have synthesized a Cas9 coding sequence with nuclear localization signals (nls) at both its amino and carboxyl termini and codons optimized for zebrafish expression (nlszCas9-n

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    Source URL: www.addgene.org

    Language: English - Date: 2014-11-05 23:32:00
    20Chemistry / Polymerase chain reaction / DNA sequencing / Sequencing / Helicase-dependent amplification / Biology / Molecular biology / Biochemistry

    V. Preparation of Sequencing Template for Plasmids. A. TempliPhi DNA Amplification

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    Source URL: nematol.unh.edu

    Language: English - Date: 2008-05-24 20:29:15
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