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Date: 2013-11-01 11:39:14 Molecular biology Biotechnology Emerging technologies Enterobacteria Escherichia coli Plasmid Synthetic biology Genetic engineering Ori Biology Bacteria Microbiology		Add to Reading List Source URL: cs.wellesley.edu Download Document from Source Website File Size: 194,95 KB Share Document on Facebook

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	RNAi by feeding 2 Steps 1. Transform L4440-XX plasmid into HT115 (DE3) bacteria – plate on LB/amp/tet 2. Inoculate 50 ml LB/amp/tet w. 0.5 ml O/N culture or directly from plate 3. Grow at 37°C until OD600 reaches 0.4 DocID: 1vadj - View Document