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Neuropeptide Y / Neuropeptides / Hypothalamus / Poly / Expressed sequence tag / Histology / Brain / Tissue / Distribution / Anatomy / Biology / Medicine


Brain Struct Funct DOIs00429SHORT COMMUNICATION Simplified CLARITY for visualizing immunofluorescence labeling
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Document Date: 2015-04-13 22:43:29


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File Size: 1,82 MB

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City

St. Paul / Rootstown / Indianapolis / /

Company

Draper / IgG / Millipore / Bernstein / Peninsula Laboratories / Harlan Laboratories / Bio-Rad / PerkinElmer / /

Country

United States / /

Currency

pence / /

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Facility

University of Pittsburgh / Scale bar / University of Pittsburgh Institutional Animal Care / A210 Langley Hall / Northeastern Ohio Medical University / /

IndustryTerm

hydrogel polymerization solutions / intact biological systems / hydrogel-aldehyde fixation solutions / saline solution / high-resolution imaging / hydrogel perfusing solution / fixative/hydrogel solutions / hydrogel-paraformaldehyde solution / later imaging / online version / acrylamideparaformaldehyde-acrolein crosslinking solution / hydrogel postfixation solution / histological and immunocytochemical processing techniques / acrolein-containing hydrogel solution / fixative solution / neural systems / imaging / aqueous solution / hydrogel-aldehyde fixative solution / hydrogel polymerization solution / oil layer / hydrogel monomer solution / confocal imaging session / amplified immunofluorescence labeling protocol / confocal imaging / food deprivation / passive clearing protocol / hydrogel solution / technology / present protocol / Tissue clearing solution / chemical and optical accessibility / hydrogel solutions / ionic detergent solution / mineral oil / image collection software / perfusion solution / ice-cold hydrogel solution / fixative-hydrogel monomer crosslinking solutions / storage protocol / fresh solution / /

MedicalCondition

Undernutrition / MS / /

Organization

Northeastern Ohio Medical University / University of Pittsburgh / Pittsburgh Institutional Animal Care and Use Committee / Mali PD / Department of Neuroscience / University of Pittsburgh Institutional Animal Care / /

Person

Ted Pella / Linda Rinaman / Jianxin Bao / H. Zheng L. Rinaman / /

/

Position

Butler / Representative / /

Product

pentobarbital sodium / glucagon / oxytocin / /

Region

Northeastern Ohio / /

Technology

Neuroscience / present protocol / CLARITY protocol / electrophoresis / antibodies / hybridization / polymerization / less expensive CLARITY protocol / passive CLARITY protocol / laser / applicable CLARITY protocol / passive clearing protocol / amplified immunofluorescence labeling protocol / Animals Experimental protocols / storage protocol / excited using a 638 nm Diode laser / excited using a 552 nm OPSL laser / /

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