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151	Synthetic Biology CAREER FAIR at the iGEM 2015 Giant Jamboree September 27, Hynes Convention Center - Boston, MA As part of the iGEM 2015 Giant Jamboree weekend, iGEM is hosting a career fair event to foster relat Add to Reading List Source URL: 2015.igem.org Language: English - Date: 2015-04-29 10:12:17 IGEM Biotechnology Science Biology Synthetic biology Genetic engineering
152	Desaturase genes PCR Protocol: (a). Using TaKaRa Ex Taq™ kit 1. Prepare 50 µl reaction in 0.5 ml PCR tube on ice Volume Add to Reading List Source URL: 2014.igem.org Language: English - Date: 2014-09-24 09:55:14 Chemistry Polymerase chain reaction Laboratory techniques Biotechnology Amplifiers Primer Thermal cycler Nucleic acid thermodynamics Variants of PCR Biology Molecular biology Biochemistry
153	Guide to Depositing Synthetic Biology Parts When depositing your standard parts with Addgene, we encourage you to include some additional information which will make it easier for requesting scientists to use them in fut Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-18 11:27:50 Microbiology Gene delivery Biotechnology Plasmid BioBrick IGEM Biology Synthetic biology Molecular biology
154	Protocols Restriction and Ligation Restriction Digestion (Adjusted protocol based on New England Biolabs NEB Biobrick Assembly Kit protocol) Add to Reading List Source URL: 2014.igem.org Language: English - Date: 2014-10-13 16:37:59 DNA ligase Insert Restriction enzyme Restriction digest BioBrick Molecular biology Biology Biochemistry
155	Colony PCR analysis Protocol: 1. Add 20 μL of sterilized distilled water into each PCR tube. 2. Pick colonies with a sterile pipette tip and resuspend a colony in a PCR tube. 3. Bacterial cells were lysed by heating at Add to Reading List Source URL: 2014.igem.org - Date: 2014-10-02 03:13:03
156	FavorPrep TM Plasmid DNA Extraction Mini Kit protocol. Add to Reading List Source URL: 2014.igem.org Language: English - Date: 2014-10-02 03:13:26 Chemistry Biology Molecular biology Centrifuges Laboratory centrifuge Laboratory glassware Centrifugation Plasmid preparation Laboratory techniques Unit operations Science
157	Preparation of LB plates Approximate formula (per liter) of Luria Broth Agar Base (Difco) Pancreatic Digest of Casein 10.0 g Add to Reading List Source URL: 2014.igem.org - Date: 2014-10-02 03:13:20
158	‘tesA PCR Protocol: (a). Using TaKaRa Ex Taq™ kit 1. Prepare 50 µl reaction in 0.5 ml PCR tube on ice Volume Add to Reading List Source URL: 2014.igem.org Language: English - Date: 2014-09-16 03:03:52 Molecular biology Science Laboratory techniques Biotechnology Hoffmann-La Roche Thermal cycler Variants of PCR Biology Polymerase chain reaction Chemistry
159	Brand: FAVORGEN BIOTECH CORP. Kit: FavorPrep GEL/PCR Purification Kit PCR Clean-Up Protocol: 1. Mix 100 μL of PCR product mixture with 5 volumes of FADF Buffer in a Add to Reading List Source URL: 2014.igem.org Language: English - Date: 2014-10-02 03:13:32 Biology Molecular biology Biotechnology Polymerase chain reaction Hoffmann-La Roche Laboratory centrifuge Centrifuge Laboratory techniques Chemistry Science
160	iGEM 2014 Measurement Interlab Study Worksheet To participate in this study, please complete the following worksheet and submit to measurement [AT] igem [DOT] org. Inter-Lab Study Worksheet: Add to Reading List Source URL: 2014.igem.org Language: English - Date: 2014-10-15 07:25:18 Biochemistry BioBrick Polymerase chain reaction Relative fluorescence units Gene expression Fluorescence Molecular biology Biology Chemistry

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