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Galactosides / Catalysis / Hydrolases / Beta-galactosidase / Enzyme assay / Enzyme kinetics / X-gal / Chemical biology / Assay / Chemistry / Biology / Enzymes


A nanostructure-initiator mass spectrometry-based enzyme activity assay Trent R. Northen*, Jinq-Chyi Lee†, Linh Hoang*, Jason Raymond‡, Der-Ren Hwang†, Steven M. Yannone§, Chi-Huey Wong†¶, and Gary Siuzdak*¶ *
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Document Date: 2013-12-10 15:01:36


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City

Tempe / La Jolla / Fluorous / Livermore / Merced / Berkeley / /

Company

EMD Biosciences / Fisher Scientific / Lawrence Livermore National Laboratory / Lawrence Berkeley National Laboratory / Bio-Rad / PerkinElmer / /

Country

United States / /

Currency

pence / /

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Facility

U.S. Department of Energy Joint Genome Institute / University of Illinois / Arizona State University / The Skaggs Institute / University of California / The Scripps Research Institute / /

IndustryTerm

sample solution / widespread applications / chemical synthesis / chemical transformations / unmodified reaction products / enzyme solutions / stock protein solution / Step-bystep protocols / stop solution / reaction products / enzymatic products / galactosidase solutions / chemical biology / local alignment search tool / e-value / pharmaceutical research / important applications / stock solutions / substrate solution / enzyme solution / gas phase / identifying the unmodified reaction products / /

NaturalFeature

Yellowstone / Sargasso Sea / /

Organization

University of California / University of Illinois / Chicago / Molecular Biology and Scripps Center for Mass Spectrometry / National Academy of Sciences / Department of Chemistry / School of Natural / Department of Molecular Biology / U.S. Department of Energy Joint Genome Institute / Arizona State University / Skaggs Institute for Chemical Biology / U.S. Department of Energy office of Science / Scripps Research Institute / NHS / /

Person

Jason Raymond / Gary Siuzdak / Chi-Huey Wong / Steven M. Yannone / Trent R. Northen / /

Position

T.R.N. / enzymatic activity Author / representative / /

Product

calcium chloride / /

ProvinceOrState

Illinois / California / /

PublishedMedium

Molecular Biology / /

Technology

Genomics / NIMS technology / S/N / 2.5-cm chip / high-throughput screening / cloning / NIMS chips / CMP / gene cloning / laser / Step-bystep protocols / Drug discovery / biofuels / DNA Chip / spectroscopy / /

URL

http /

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