Human Microbiome Project - Document - PDFSEARCH.IO

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Date: 2014-08-13 16:13:29 Bacteriology Environmental microbiology Health Human Microbiome Project Human microbiome Gut flora Microbiome Pathogenic bacteria Microorganism Microbiology Bacteria Biology		Add to Reading List Source URL: www.cihr-irsc.gc.ca Download Document from Source Website File Size: 543,86 KB Share Document on Facebook

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	RNAi by feeding 2 Steps 1. Transform L4440-XX plasmid into HT115 (DE3) bacteria – plate on LB/amp/tet 2. Inoculate 50 ml LB/amp/tet w. 0.5 ml O/N culture or directly from plate 3. Grow at 37°C until OD600 reaches 0.4 DocID: 1vadj - View Document