CRISPR

Results: 222



#Item
131Genetics / Molecular genetics / DNA ligase / DNA replication / Guide RNA / Nuclear localization sequence / Transformation / DNA / Ligation / Biology / Molecular biology / RNA

CRISPR plasmids from Chen and Wente labs We have synthesized a Cas9 coding sequence with nuclear localization signals (nls) at both its amino and carboxyl termini and codons optimized for zebrafish expression (nlszCas9-n

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Source URL: www.addgene.org

Language: English - Date: 2014-11-05 23:32:00
132Genetics / CRISPR / Non-coding RNA / Genetic engineering / Genetically modified organisms / Gene targeting / Biology / Bioengineering / Molecular biology

Double-nicking CRISPR/Cas9: precise modification of the mouse genome with greatly reduced off-target effects David Zahra1, Kevin Taylor2, Jana Hermes1, Jenny Kingham2 and Robert Brink1,3 1Garvan Institute of Medical Res

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Source URL: www.towardsprecisionmedicine-symposium2014.org.au

Language: English - Date: 2014-09-23 20:24:49
133Genetic engineering / Biology / CRISPR / Non-coding RNA

Rapid generation of mutant mice using TALEN- and CRISPR-mediated genome editing Fatwa Adikusuma, Sandra Piltz, Daniel Pederick, Ella Thomson, Ruby Moffat, James Hughes, & Paul Thomas Discipline of Biochemistry, Universit

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Source URL: www.towardsprecisionmedicine-symposium2014.org.au

Language: English - Date: 2014-05-29 18:46:36
134Microbiology / Caudovirales / Lambda phage / Genome / CRISPR / Virus / Brucella / Lytic cycle / Myoviridae / Biology / Bacteriophages / Bacteria

Whole genome sequence comparison of ten diagnostic brucellaphages propagated on two Brucella abortus hosts

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Source URL: www.virologyj.com

Language: English
135Genomics / Genetic mapping / Guide RNA / RNA / Molecular biology / Genome engineering / CRISPR / Human genome / Genome / Genetics / Biology / Bioinformatics

Identification of high-efficiency 3’GG gRNA motifs in indexed FASTA files with ngg2 1 PrePrints

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Source URL: peerj.com

Language: English - Date: 2015-04-08 18:10:41
136CRISPR / Molecular genetics / Non-coding RNA / Virus / Nucleic acid / Immune system / DNA / Bacteria / Gene / Biology / Genetics / RNA

Researchers report on CRISPR-cas surveillance complex that targets RNA

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Source URL: phys.org

Language: English - Date: 2015-05-04 01:56:17
137CRISPR / Non-coding RNA / Gene / Molecular genetics / Biology / Genetics / Molecular biology

Altering genes with the aid of light

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Source URL: phys.org

Language: English - Date: 2015-05-17 21:31:30
138RNA / Molecular biology / Genetic mapping / CRISPR / Genome engineering / Zinc finger nuclease / Guide RNA / Non-coding RNA / DNA / Biology / Genetics / Genomics

Report fluorescent. Homologous recombination (HR) using an appropriate repair donor can restore the normal GFP sequence, enabling us to quantify the resulting GFP+ cells by flow activated cell sorting (FACS). To test the

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Source URL: arep.med.harvard.edu

Language: English - Date: 2013-01-04 11:15:13
139Biotechnology / Emerging technologies / Bioethics / Gene therapy / CRISPR / Mary Ann Liebert /  Inc. / Human genome / Biology / Genetics / Molecular biology

New gene editing tools force renewed debate over therapeutic germline alteration

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Source URL: medicalxpress.com

Language: English - Date: 2015-05-16 01:13:33
140RNA / Microbiology / CRISPR / Lac operon / Transformation / Isopropyl β-D-1-thiogalactopyranoside / Non-coding RNA / Gene expression / Molecular cloning / Biology / Molecular biology / Genetics

Supplemental Information tracrRNA cas9 cas1

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Source URL: limlab.ucsf.edu

Language: English - Date: 2014-12-24 04:42:18
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