Notes on the AAV2 vectors

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Date: 2014-11-06 19:29:41 Biochemistry BglII EcoRI SV40 HindIII BamHI Citrine Kozak Vector Biology Molecular biology Restriction enzymes		Notes on the AAV2 vectors Add to Reading List Source URL: www.tsienlab.ucsd.edu Download Document from Source Website File Size: 145,84 KB Share Document on Facebook

	pAC161-SeqFinal page < PAGE >1 of < NUMPAGES >4 Plasmid name: pAC161 Remarks: complete sequence, reanalysed on both strands T-DNA is marked in yellow (compare map) EcoRI site in map is numbered "1" in sequence DocID: 1vmQ1 - View Document
	Produce E. coli gcvR Promoter with Oligos The goal is to have the workshop make this new promoter with BioBrick ends. Will use oligo assembly method. Desired sequence: Need biobrick prefix end with EcoRI sticky end -35 DocID: 1v1aa - View Document
	EcoRI (396bp) VCre pUCNLS-VCre (3911bp) DocID: 1uwZx - View Document
	EcoRI (396bp) SacI (406bp) KpnI (412bp) VloxP MluI (482bp) NotI (489bp) DocID: 1tSTi - View Document
	DNA purification with SigmaSpin post-reaction cleanup-plates required: centrifuge capable of spinning 96 well plates (we used a Heraeus 3SR) dont forget: after reaction, before cleanup: heat-inactivate encyme: e.g. EcoRI DocID: 1s84a - View Document