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51	CRISPR plasmids from Chen and Wente labs We have synthesized a Cas9 coding sequence with nuclear localization signals (nls) at both its amino and carboxyl termini and codons optimized for zebrafish expression (nlszCas9-n Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:00 Genetics Molecular genetics DNA ligase DNA replication Guide RNA Nuclear localization sequence Transformation DNA Ligation Biology Molecular biology RNA
52	Choice of gRNAs for the Cas9 Orthologs S. pyogenes Cas9 (SP): Find all 23bp genomic sites of the form 5’-NNNNNNNNNNNNNNNNNNNNNGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – st Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:31:59 Molecular biology Genetics DNA Gene expression RNA Biology Guide RNA
53	Op en Source W nt Project Gateway Vectors: You have received all 19 human Wnt clones in two Gateway vector backbones. The first set of clones are in “Entry” vectors, and the second set are in “Destination” vector Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:03 Protein biosynthesis Gene expression Molecular genetics Gene delivery Wnt signaling pathway Vector Open reading frame Expression vector Stop codon Biology Biochemistry Molecular biology
54	gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’ Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:02 Chemistry Guide RNA Polymerase chain reaction Oligonucleotide Molecular cloning Vector DNA microarray Gene expression Biology Molecular biology Biochemistry
55	Fire Lab 1999 Vector Supplement- Maygfp color variants Some new localization signals RNA-interference controls Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:09 Gene expression Gene delivery Genetics DNA Green fluorescent protein Vector Reporter gene Expression vector Intron Biology Biochemistry Molecular biology
56	Ordering from Addgene Ordering FAQs All orders must be placed at www.addgene.org Add to Reading List Source URL: www.addgene.org Language: English - Date: 2015-02-09 12:47:12 Gene delivery Microbiology Molecular biology Plasmid Invoice Point of sale Order Purchasing Business Biology Electronic commerce
57	Wnts in pENTR/D-TOPO Map           Add to Reading List Source URL: www.addgene.org - Date: 2014-11-05 23:32:07
58	Wnts in pcDNA3.2/V5-DEST Map         Add to Reading List Source URL: www.addgene.org - Date: 2014-11-05 23:32:00
59	FireLab 2005 Mini-vector Kit July 2005 Documentation "alpha" version Direct contributors to making and testing vectors in this kit In our lab: Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:08 Vector Green fluorescent protein Expression vector Plasmid Restriction enzyme Multiple cloning site Molecular cloning Restriction digest Gene expression Biology Molecular biology Biochemistry
60	RNAi info from the Fire Lab VersionFebruary 1998 Contents: I. A very short introduction to RNA-mediated interference II. Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:08 Gene expression RNA interference Caenorhabditis elegans Andrew Fire Sense Gene silencing Craig Mello Oligonucleotide Small interfering RNA Genetics Biology RNA

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