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#	Item
41	Open Source Wnt Nomenclature - Wnt1-STOP: Wnt1 sequence with a STOP codon cloned into the Entry Vector, pENTR/D-TOPO - Wnt1-NONSTOP: Wnt1 sequence without a STOP codon cloned into the Entry vector pENTR/D-TOPO - Wnt1 (un Add to Reading List Source URL: www.addgene.org - Date: 2014-11-05 23:32:04
42	iChIP / enChIP to purify genomic DNA Originally developed by Toshitsugu Fujita on September 18, 2012 Modified by Hodaka Fujii on December 29, Crosslinking of cells (1) Culture target cells. Use 2 x 107 cells (e.g Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:31:54 Laboratory techniques Cell biology Protein methods Lysis buffer Litre Buffer solution Immunoprecipitation Ethylenediaminetetraacetic acid Tris Chemistry Buffers Amines
43	Bacteria-mediated RNAi--General outline: Lisa Timmons Carnegie Institution of Washington Updated MayPlace gene of interest between T7 promoters in “double T7” plamsid. (slightly more difficult alternative: P Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:31:55 Microbiology Gene expression Mobile genetic elements Isopropyl β-D-1-thiogalactopyranoside Transformation Lac operon RNA interference Tetracycline Plasmid Biology Molecular biology Gene delivery
44	C. elegans miniMos Transgenesis Kit Description: The miniMos transposon is a modified Mos1 transposon that can be used to generate transgenic nematodes, including C. elegans and C. briggsae. The Mos1 transposon has been Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:02 Microbiology Gene delivery Plasmid Laboratory equipment Transgenesis Pipette Biology Mobile genetic elements Molecular biology
45	Fire Lab Vector Kit- Juneupdated to AugustCredits: Making & Testing New Vectors: Si-Qun Xu Vector Design, Strategic Mistakes, Late Night Gel Photos: Andy Fire Analysis of mRNA localization, lacZ in early em Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:31:55 Gene expression RNA splicing Spliceosome Genetics Vector Green fluorescent protein Enhancer Reporter gene Lac operon Biology Biochemistry Molecular biology
46	FREQ-Seq kit Description: Tracking the frequencies of specific alleles in microbial populations through time is an important aspect for studying evolutionary dynamics. The FREQ-Seq collection and accompanying documentati Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:09 Plasmid Pipette Sequencing Biology Molecular biology Biochemistry
47	75 Sidney St, Suite 550A, Cambridge, MA 02139 • Tel: • Fax: Instructions for Online Plasmid Submission 1. Log into your account on Addgene’s website. 2. For each plasmid, Add to Reading List Source URL: www.addgene.org Language: English - Date: 2015-03-17 14:55:29 Mobile genetic elements Gene delivery Plasmid Biology Molecular biology Microbiology
48	Golden Gate TALEN and TAL Effector Kit 2.0 Description: TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nucl Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:03 Molecular biology Microbiology Gene delivery Mobile genetic elements Plasmid TAL Pipette Effector Biology Proteins TAL effector
49	cpFRET Biosensor Toolkit Description: The circular permutation-based fluorescence resonance energy transfer (cpFRET) biosensor toolkit is a vector library for the generation and/or optimization of genetically encoded, un Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:31:53 Mobile genetic elements Biotechnology Gene delivery Plasmid Biosensor Pipette Georg Heinrich Pertz Biology Molecular biology Microbiology
50	Wolfe-Lawson ZFN modular assembly kit Description: Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. We provided an archive of 81 clones containing 27 different zinc-finger Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:03 Protein domains Molecular biology Laboratory equipment Zinc finger nuclease Zinc finger Plasmid Pipette Zinc Agar plate Biology Chemistry Biochemistry

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