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21	Genome-scale CRISPR Knock-Out (GeCKO) v2.0 pooled libraries CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a microbial nuclease system containing a combination of CRISPR-associated (Cas) genes a Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:31:53 Gene delivery Genetics Molecular genetics Microbiology CRISPR Plasmid Transformation Viral vector Vector Biology Molecular biology Biochemistry
22	LentiCRISPRv2 and lentiGuide-Puro: lentiviral CRISPR/Cas9 and single guide RNA CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a microbial nuclease system involved in defense against invading Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-18 13:31:05 CRISPR Viral vector Plasmid Vector Transformation Lentivirus RNA Molecular cloning Biology Molecular biology Gene delivery
23	Fire Lab 1997 Vector Supplement- Februarygfp variants PCR-based gfp tagging restriction-site-based gfp tagging gfp intron-tagging Add to Reading List Source URL: www.addgene.org Language: English - Date: 2011-07-16 08:53:32 Green fluorescent protein Vector Gene expression Restriction enzyme Transcription RNA splicing Intron Polymerase chain reaction Mosaic Biology Molecular biology Biochemistry
24	PDF Document Add to Reading List Source URL: www.addgene.org - Date: 2011-07-16 00:28:42
25	PDF Document Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:05 Gene delivery Microbiology Mobile genetic elements Plasmid Vector Pipette Biology Molecular biology Biochemistry
26	PDF Document Add to Reading List Source URL: www.addgene.org - Date: 2015-03-12 10:55:49
27	Sequencing TALENs Sequencing TALENs (or any TAL array) can be challenging due to the repetitive nature of the sequence. In the Voytas lab, we usually are only concerned with the identity of the RVDs in each repeat as the Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:31:59 Chemistry Sequencing Primer Polymerase chain reaction Nucleic acid sequence Biology Molecular biology Biochemistry
28	Golden Gate TALEN assembly This is an expanded and slightly modified TAL assembly protocol published in the original form in Cermak, et al., 2011 (http://dx.doi.orgnar/gkr218) Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:03 Plasmid Molecular cloning Restriction enzyme Vector TAL effector Restriction digest Polymerase chain reaction Gateway Technology Subcloning Biology Molecular biology Biochemistry
29	Human Kinase Open Reading Frame Collec6on Description: The Center for Cancer Systems Biology (Dana Farber Cancer Institute)Broad Institute of Harvard and MIT Human Kinase ORF collection from Addgene consists of Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:08 Protein kinases Cell cycle Molecular biology Tyrosine kinase receptors Plasmid TIE1 Cyclin-dependent kinase AKT Mitogen-activated protein kinase Biology Cell biology Proteins
30	Construct Design and Cloning Guide for Cas9-triggered homologous recombination Written by Dan Dickinson () and last updated DecemberReference: Dickinson DJ, Ward JD, Reiner DJ and Goldstein B Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-19 10:38:34 Molecular biology Cre recombinase Homologous recombination Genetic recombination Site-directed mutagenesis Gene Cre-Lox recombination Recombineering Biology Genetics Molecular genetics

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