NDA[removed]S-022 Page 3

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Date: 2009-03-31 14:11:39 Carbapenem antibiotics Beta-lactam antibiotics Pyrrolidines Meropenem Klebsiella pneumoniae Beta-lactamase Vancomycin Carbapenem Cephalosporin Bacteria Microbiology Gram-negative bacteria		NDA[removed]S-022 Page 3 Add to Reading List Source URL: www.accessdata.fda.gov Download Document from Source Website File Size: 193,59 KB Share Document on Facebook

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	RNAi by feeding 2 Steps 1. Transform L4440-XX plasmid into HT115 (DE3) bacteria – plate on LB/amp/tet 2. Inoculate 50 ml LB/amp/tet w. 0.5 ml O/N culture or directly from plate 3. Grow at 37°C until OD600 reaches 0.4 DocID: 1vadj - View Document